Journal: The EMBO Journal

Article Title: Ca²⁺ leakage is a conserved signal for non-canonical ATG8/LC3 lipidation and membrane repair

doi: 10.1038/s44318-026-00741-z

Figure Lengend Snippet: ( A ) STED super-resolution imaging of THP-1 macrophages stably expressing Halo-LC3, labelled with Halo dye, under 10 min LLOMe treatment. White arrowheads indicate the LC3-TVS underwent dynamic fusion. ( B – D ) Time-lapse images showing membrane remodelling of LC3-positive vesicles induced by lysosomal damage. THP-1 macrophages stably expressing GFP-LC3B were under normal condition or treated with LLOMe. Cells were imaged using VT-iSIM super-resolution microscopy at 15-second intervals. Images are z-maximum projections covering entire cells (0.5 µm per stack, 5–7 µm total). Time 0 indicates the frame acquired at the onset of LLOMe treatment, following a 10-min stabilization period. LC3-positive structures under untreated conditions ( B ). Representative regions of interest (ROIs) showing dynamic LC3-positive vesicles from three independent experiments (related to Movie ) under LLOMe treatment ( C , D ). Yellow, pink, and white arrows indicate distinct docking and fusion events observed over time. ( E ) Quantification of percentage of single-, double-, and multiple-membrane RFP⁺GFP⁺ LC3-positive vesicles relative to the total number of RFP⁺GFP⁺ LC3-positive vesicles determined by CLEM ( n = 3 cells, 167 vesicles were analysed), related to Fig. and F–H. ( F ) CLEM analysis showing RFP-LC3 and GFP-LC3 double-positive multimembrane structures following LLOMe treatment (related to Fig. and Movie ). Yellow arrowhead indicates the LC3-positive multimembranes. ( G , H ) CLEM analysis reveals complex membrane structures positive for both RFP-LC3 and GFP-LC3 after LLOMe treatment. Yellow arrowheads indicate the LC3-positive multimembranes. Pink arrowheads indicate the LC3-positive single membrane. Scale bars: ( A – D ), 1 μm; ( F – H ), 1 μm (main images), 200 nm (zoomed-in areas).

Article Snippet: Cells were imaged using a VT-iSIM superresolution imaging system (Visitech International) with an Olympus IX83 microscope, or a Leica STELLARIS 5 confocal microscope equipped with an environmental chamber (Okolab) maintained at 37 °C with 5% CO2.

Techniques: Imaging, Stable Transfection, Expressing, Membrane, Super-Resolution Microscopy

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Myosin Va transport of liposomes in three-dimensional actin networks is modulated by actin filament density, position, and polarity

doi: 10.1073/pnas.1901176116

Figure Lengend Snippet: Myosin Va teams transport intracellular cargo through networks of actin. (A) Lipid-bound cargo is produced and packaged at the interior of the cell within the Golgi. These cargos are first transported along microtubule tracks, followed by handoff to MyoVa for distribution and final delivery to sites of secretion at the cell membrane. (B) Superresolution, 3D STORM reconstruction of an in vitro actin network. Actin filaments are strung between silica beads of varying diameters, which support the network and maintain a 3D organization. Color represents z position. (Scale bar: 2 µm.) (C) Overlay of 350-nm vesicle trajectory (magenta) by teams of MyoVa within a 3D actin filament network (colored by z position). (Scale bar: 1 µm.)

Article Snippet: 3D STORM images were acquired using a Nikon N-STORM superresolution microscope system with excitation of Alexa-647 phalloidin-labeled actin by 647- and 405-nm lasers.

Techniques: Produced, In Vitro